Organization

Boston Children's Hospital

Location

Boston, Massachusetts

Date Posted

August 27, 2021

Job Categories

Full Time

At Boston Children’s Hospital, the quality of our care – and our inclusive hospital working environment – lies in the diversity of our people. With patients from local communities and 160 countries around the world, we’re committed to reflecting the spectrum of their cultures, while opening doors of opportunity for our team. Here, different talents pursue common goals. Voices are heard and ideas are shared. Join us, and discover how your unique contributions can change lives. Yours included.

Project Description:
The Research Assistant  will be involved in the generation of knockout zebrafish lines and will work alongside a postdoctoral research fellow to characterize this zebrafish larvae using a variety of techniques including PCR, qPCR, western blotting, CRISPR-mediated gene editing, RNA sequencing, immunohistochemistry, microscopy and automated analyses of motor behavior.
The Research Assistant will maintain genetically-modified zebrafish lines. Methods include the use of CRISPR-Cas9, stereotactic oocyte injections, genotyping and zebrafish husbandry. Basic design of experiments and statistics will be taught or current skills enhanced.

This Research Assistant will also be responsible for:

  • According to prescribed protocols and assigned schedules, performs a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy.
  • Assists in troubleshooting procedures if experimental results are unexpected or quality of specimens is poor.
  • Maintains records of results and methodologies in a log of work performed.
  • Maintains equipment, lab area and supplies in a neat professional manner.
  • Sterilize and clean glassware, prepare media and other materials using appropriate procedures.
  • Inventories supplies alerts supervisor to needed supplies, or mixes standard reagents.
  • Processes order for supplies and/or animals. Attends courses in safety procedures for handling, storage and disposal of biohazardous, flammable, corrosive and toxic materials.
  • Carries out all laboratory activities in compliance with applicable regulations and protocols.
  • Conducts literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I.
  • Attends courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information.
  • Contributes to the team within and between departments and organizations.

To qualify, you must have:

  • The knowledge of theories, principles, and concepts acquired through completion of a Bachelor of Science degree and one year of post grad relatable experience.
  • Analytical skills to resolve problems that require the use of basic scientific, mathematical, or technical principles.
  • Ability to communicate effectively both orally and in writing
  • Prior experience with common molecular biology techniques and/or handling zebrafish is preferred but not required

Please note: During a public health emergency, individuals in this role may be expected to take on additional duties to respond to organizational needs.

Boston Children’s Hospital offers competitive compensation and unmatched benefits, including a rotating days/evenings and week-end schedule, affordable health, vision and dental insurance, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition Reimbursement, cell phone plan discounts and discounted rates on T-passes (50% off). Discover your best.

Responsibilities

Qualifications

Application Process

Apply Here: https://sjobs.brassring.com/TGnewUI/Search/home/HomeWithPreLoad?PageType=JobDetails&partnerid=368&siteid=5205&Areq=54699BR#jobDetails=3189171_5205


About the Organization

Boston Children’s Hospital is dedicated to improving and advancing the health and well-being of children around the world through its life-changing work in clinical care, biomedical research, medical education and community engagement.

Boston Children’s is ranked the number one pediatric hospital in the nation by U.S. News and World Report. It is home to the world’s largest pediatric research enterprise, and it is the leading recipient of pediatric research funding from the National Institutes of Health. It is the primary pediatric teaching hospital for Harvard Medical School. Boston Children’s treats more children with rare diseases and complex conditions than any other hospital.

Boston Children’s is dedicated to creating a culture where all patients, families, clinicians, researchers, staff, and communities feel empowered and supported. We are committed to working together to support health equity and promote anti-racist practices. This is not merely an aspirational goal, and in August 2020, we shared our formal Declaration on Equity, Diversity and Inclusivity that will be our guiding compass in making this goal a reality.

With more than 40 clinical departments and 258 specialized clinical programs, Boston Children’s is one of the largest pediatric medical centers in the United States.

Contact Name

Thamar Fedestin

Contact Phone

6173551908

Contact Email

thamar.fedestin2@childrens.harvard.edu

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